The human protein atlas blog


A subcellular map of the human proteome

2017-05-12
Cell Atlas Immunocytochemistry Immunofluorescence Organelle Science

30 organelles and substructures are annotated in the Cell Atlas.

The Cell Atlas database was just recently released with data on more than 12.000 proteins and mapping to 30 organelles. Today, the scientific findings are published in Science in "A subcellular map of the humanproteome".

The cell is a complex entity that carries out multiple functions. In order to do this, the different parts of the cells are organized in structures, called organelles. By investigating the organelles and its proteome, and to understand how the proteins vary over time and space we can truly begin to understand human biology on a detailed level...Read more


Cell Image of the Month – MCM6

2017-02-24
Cell Atlas Image of the week Immunofluorescence Lactase

In 2017, February 28th will be the day that in Sweden is known as "Fettisdagen", (lit. "Fat Tuesday"), in other countries known as "Mardi Gras", "Faschingsdienstag" or "Shrove Tuesday". This day was originally celebrated in the Christian tradition as the last day of a three day feast to prepare for the forty day long fasting period before Easter. Today it has been popularized and in many places around the world this day is now known for its carnivals.

In Sweden together with some other northern European countries we like to eat "semlor" on Fat Tuesday...Read more


Validation of antibodies for bioimaging

2017-01-31
Antibody Antibody Validation Bioimaging Immunofluorescence

Marie Skogs, first author of the study

In a recent publication in Journal of Proteome Research, researchers from the Cell Profiling group of the Human Protein Atlas team present a new approach for validation of antibodies for bioimaging applications.

Antibodies are indispensible research tools, yet the scientific community has not adopted standardized procedures to validate their specificity. Here the researchers present a strategy to systematically validate antibodies for immunofluorescence applications using gene tagging.

Marie Skogs is the first author of the study.

– After a M. Sc...Read more


Sample preparation – planning is crucial

2016-11-22
Antibody Cell Atlas Immunofluorescence

Christian Gnann by the Tecan's Freedom EVO pipetting robot

To prepare all the images for the Cell Atlas, released on December 4, the cells used are primed for staining and microscopy. Sample preparation is an important step when performing immunofluorescence studies. If wrongly applied it can not only cause unsuccessful detection but also generate misleading information.

The sample preparation performed by the Cell Atlas team includes cell fixation, permeabilization, and immunostaining with primary and secondary antibodies.

– The fixation is the crucial step, and different fixation protocols work better for different sets of proteins, Christian Gnann, a research engineer in the Cell Profiling group explains...Read more


Culturing cells for the Human Cell Atlas

2016-11-15
Cell Atlas Immunofluorescence

Anna Bäckström with the cells in a LAF bench

The Human Cell Atlas, to be released on December 4, displays high resolution, multicolour images of immunofluorescently stained cells. This provides spatial information on protein expression patterns on a fine cellular and subcellular level. From the start three cell lines, U-2 OS, A-431 and U-251 MG, originating from different human tissues were chosen to be included in the immunofluorescent analysis...Read more